Primer Design (PRIDE 2.0)
PRIDE is a primer design program that automatically designs primers in single contigs or whole sequencing projects to extend the already known sequence and to double strand single-stranded regions. The program is fully integrated into the Staden package (GAP4).
More details about PRIDE as well as about additional tools you will find in the manual.
A less automated version of PRIDE which can only be used for the design of primers on a single sequence is presented at http://www.dkfz-heidelberg.de/tbi/services/Pride/search_primer
minimal set of user-defined parameters
ability to check for secondary binding sites within vector or repetitive elements
fast computation of primers also on complicated sequences (e.g. high GC-content)
already designed primers are recognized
visualization of primers by specific tags
primers are named uniquely according to the template they are designed on
flexible naming convention by using regular expressions
additional tools for displaying and removal of groups of annotation tags
Availability and Compatibility
Last modified Apr 2006 by Stefan Haas